ABSTRACT
Objective To investigate the relationship between tenascin-C (TNC) and acute rejection (AR) early after liver transplantation. Methods Six Brown Norway (BN) rats and 16 Lewis rats were divided into the AR group (Lewis→BN, 6 donors and 6 recipients) and control group (Lewis→Lewis, 5 donors and 5 recipients). The transplant liver tissues from rats in two groups were subjected to pathological examination. The rejection activity index (RAI) was evaluated by Banff schema. The expression of TNC proteins in the transplant liver tissues were determined by immunohistochemical staining and Western blot. The expression level of serum TNC was detected by enzyme-linked immune absorbent assay (ELISA), and the correlation between TNC and RAI score was analyzed. Results Pathological examination of the transplant liver at 7 d after liver transplantation showed that the RAI score in the AR group was higher than that in the control group. Immunohistochemical staining results found that the distribution of TNC positive cells of the transplant liver in the AR group was more than that in control group at postoperative 7 d. Western blot showed that the relative expression level of TNC protein in the AR group was significantly higher than that in the control group at 7 d after liver transplantation (t=5.112, P=0.007). ELISA results revealed that the serum TNC expression level in the AR group was significantly higher than that in the control group at 7 d after liver transplantation (t=3.152, P=0.012). The serum TNC expression level was positively correlated with the RAI score (r=0.790 9, P=0.004). Conclusions The expression level of TNC is associated with AR after liver transplantation. TNC may become a novel target for diagnosis and treatment of AR early after liver transplantation.
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@#ObjectiveIn order to study the effect of three-month rehabilitation on motor function in hemiplegics after stroke. Methods125 patients were randomly divided into two groups, rehabilitation group (72 cases)and control group(53 cases).Patients in the rehabilitation group were given clinical treatment,electric stimulation therapy(EST) and regularly physical therapy, while those in control group were given clinical treatment and unguided self-training, and the evaluation was done in pre-treatment and post-three-month respectively. Motor function was assessed in Fugl-Meyer Assessment(FMA).ResultsMotor scores in each group had a more significant difference(P<0.001)before and after treatment, the process of scores in the rehabilitation group was obviously superior to that in the control group(P<0.001). Conclusions Rehabilitation training on hemiplegics after stroke may obviously improve motor function, depressing the disability and increasing the living quality.
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Object To determine the content of resveratrol glucoside and resveratrol in radix and rhizome of Polygonum cuspidatum Sieb. et Zucc. yielded in Hanzhong Region. Methods The HPLC method was used to assay resveratrol glucoside and resveratrol directly, using Eclipse XDB C 8 column and acetonitrile water as the mobile phase, the UV detection wavelength was 303 nm, with a flow rate of 1 mL/min. Results The content of resveratrol glucoside was 2 5% and resveratrol was 0 43% in the radix and rhizome. Conclusion The content of resveratrol glucoside is higher than that in the former refernece. The content of resveratrol in the radix and rhizome of P. cuspidatum in Hanzhong Region is first reported.
ABSTRACT
The clamp-on shapping plate implant, composed of a main rod with 3～4 pairs of curved short arms attached on both sides of the rod, possesses multifixing action to the fracture without hindering the movement of the adjacent joints. It is especially useful for the fixation of comminuted fracture of the short tubural bones which are difficult to be fixed by other methods. In the period of 1987.8～1990. 2,64 cases of such fractures had been treated with this device. Followup results in 52 cases showed that all the fractures thus treated healed nicely without any case of malunion. Total duration of external immobilization was relatively shorter and the functional recovery of the joints also much better.